School Of Science And HealthAnalytical Protein ScienceWestern Sydney University Unit Code: 300556.2
Discipline: BIOCHEMISTRY AND CELL BIOLOGY
Student Contribution Band: 2
Level: 3
Credit Points: 10
Assumed Knowledge
Detailed knowledge of protein structure at primary, secondary, tertiary, and quaternary levels; relationship between protein structure and protein function; protein denaturation; isoelectric points and their relevance for protein separation; basic knowledge of and competency in biochemical laboratory techniques, such as spectrophotometry, centrifugation, enzyme assay, graphical methods and biochemical calculations.
Equivalent Units
J3656 - Analytical Biochemistry
Prerequisite
300219 Biochemistry 1 OR 300555 Proteins and Genes
About this Unit
This unit covers a range of biochemical techniques and methodologies used for both analysis and purification of biological molecules. It includes advanced aspects of spectroscopy, centrifugation, radioisotopes; RNA isolation and detection, chromatographic principles and methods (gel filtration, ion exchange, affinity, hydrophobic interaction, chromatofocusing); electrophoresis principles and methods (SDS-PAGE, isoelectric focusing, pore gradient, two-dimensional, capillary); protein extraction and separation strategies. The methods and applications of proteomic research are included. The laboratory work parallels lecture material, and students gain hands-on experience in many of these techniques. The importance of quality control is emphasised and quality control programs are carried out concurrently with other laboratory work.